Molecular detection of E.coli O157

This study was based on the importance of pathogens that cause diarrhea in sheep and their impact livestock, E.coli O157:H7 which are considered one most important pathogens, were studied isolated with sensitivity test during period (October 2021 to December 2021), samples taken from infected fields around Baghdad (Abu-ghraib , AL-mahmoudia AL-yosifiya ), All cultured MacConkey agar Eosine Methylene Blue Sorbitol for isolation according isolates identified by biochemical tests.out 101 samples, 100 gave positive results three appeared more resistance antibiotics isolate very sensitive antibiotics. These selected be confirmed using molecular detection PCR. Among resistant showed a diagnosis two belonging O157-H7 addition result, but similar result appear because bacteria can evolve rapidly adapt environmental change., this evolution turn harmless into harmful bacteria.